The effect of intrathecal delivery of bone marrow stromal cells on hippocampal neurons in rat model of Alzheimer’s disease

نویسندگان

  • Akram Alizadeh Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Ali Samadikuchaksaraei Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran|Department of Tissue Engineering and Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran|Department of Medical Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran
  • Babak Behnam Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran|Department of Medical Genetics and Molecular Biology, Iran University of Medical Sciences, Tehran, Iran
  • Bahareh Kordestani Shargh Department of Basic Sciences, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran
  • Mahmood Hajghasem Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Maliheh Nobakht Department of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran|Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran
  • Mansooreh Soleimani Department of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran|Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran|Department of Medical Genetics and Molecular Biology, Iran University of Medical Sciences, Tehran, Iran
  • Mina Eftekharzadeh Department of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
  • Nushin Karkuki Osguei Eposcience Millennium Institute, Tehran, Iran
چکیده مقاله:

Objective(s): Intracerebral injection of bone marrow stromal cells (BMSCs) is being investigated as a therapeutic tool to prevent Alzheimer's disease (AD). Our aim was to investigate the effects of BMSCs by intrathecal injection in AD rat model. Materials and Methods: BMSCs were obtained from the bone marrow of Wistar rat and transplanted into AD rat model via intrathecal injection. The rat model had received an injection of β amyloid into the hippocampus for histological and immunohistochemical studies. Results: Histological examination of the brains in transplanted rats compared to controls demonstrated the migration of BrdU-labeled BMSCs from the site of delivery, confirmed the differentiation of BMSCs transplanted cells into the cholinergic neurons, and increased number of healthy and decreased number of dark neurons. Conclusion: Our results showed that BMSCs intratechal administration could be a promising method for treatment ofAlzheimer’s disease in rat model.

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the effect of intrathecal delivery of bone marrow stromal cells on hippocampal neurons in rat model of alzheimer’s disease

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عنوان ژورنال

دوره 18  شماره 5

صفحات  520- 525

تاریخ انتشار 2015-05-01

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